Declarations for the John Smith's Grand National today revealed that the first 44 horses - composed of 40 runners and four reserves - have all stood their ground.
That meant that the heavily backed Whinestone Boy and well handicapped Chief Dan George were both ruled out of the contest.
The Howard Johnson-trained Abbeybraney is the last horse to make the cut.
There are four reserves - 2007 winner Silver Birch, last year's fifth Cerium, Royal Rosa and Knowhere.
They will fill any gaps should there be any non-runners announced before 9am tomorrow.
Silver Birch is also engaged to run in the John Smith's Topham Chase tomorrow, whilst Howard Jonhson has gone on record as saying that he will sacrifice Abbeybraney if it ensures that his other hope Royal Rosa gets in the Grand National line-up.